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| 2. |  | OLIVEIRA, S. M. do V. L. de; RUFFINO-NETTO, A.; PANIAGO, A. M. M.; OLIVEIRA, O. A. de; CUNHA, M. M. R. V. da; ANDREOTTI, R. Teste tuberculínico: pesquisa operacional no Mato Grosso do Sul. Jornal Brasileiro de Pneumologia, v. 37, n. 5, p. 646-654, 2011.| Biblioteca(s): Embrapa Gado de Corte. |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
18/02/2016 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
DEUS, K. E. de; LANNA, A. C.; ABREU, F. R. M.; SILVEIRA, R. D. D.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO, R. P. |
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Afiliação: |
KARINNE EVARISTO DE DEUS, UFG; ANNA CRISTINA LANNA, CNPAF; FERNANDA RAQUEL MARTINS ABREU, UFG; RICARDO DIOGENES DIAS SILVEIRA; WENDELL JACINTO PEREIRA, UFG; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Molecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v. 9, n. 8, p. 744-753, 2015. |
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ISSN: |
1835-2707 |
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Idioma: |
Inglês |
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Conteúdo: |
Drought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought tolerance; these findings may ultimately inform breeding programs aimed at more efficiently developing cultivars that are better adapted to areas prone to water deficits. MenosDrought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought toler... Mostrar Tudo |
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Palavras-Chave: |
Enzymatic activity. |
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Thesagro: |
Arroz; Oryza sativa; Resistencia a seca. |
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Thesaurus NAL: |
Gene expression; Oxidative stress; Rice; Superoxide dismutase; Water stress. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139323/1/CNPAF-2015-ajcs.pdf
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Marc: |
LEADER 02567naa a2200313 a 4500
001 2037521
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
022 $a1835-2707
100 1 $aDEUS, K. E. de
245 $aMolecular and biochemical characterization of superoxide dismutase (SOD) in upland rice under drought.$h[electronic resource]
260 $c2015
520 $aDrought is a major cause of reduced yield in upland rice farming in many regions of the world and cause an increased production of reactive oxygen species (ROS), followed by activation of a complex antioxidant system. In this study, the superoxide dismutase (SOD) enzymatic activity and the gene expression levels of eight isoforms of SOD were evaluated in drought-tolerant and drought-sensitive varieties of upland rice, Oryza sativa japonica (the Primavera and Douradão cultivars), including shoot and root tissues in two stages of plant development, grown under normal and restricted irrigation. The SOD activity was determined by spectrophotometric method and the gene expression by qPCR experiments. In the tolerant cultivar, SOD enzymatic activity increased significantly (p ≤ 0.05) only in the root tissue in the reproductive stage (268.00 SOD UN mg-1), whereas in the sensitive cultivar, SOD increased in the leaf (112.17 SOD UN mg-1) and root (172.56 SOD UN mg-1) tissues in the reproductive stage. The genes CuZnSOD4 and MnSOD showed significant increases in expression (p ≤ 0.05) in both tissues and developmental stages in the tolerant cultivar under drought, whereas FeSOD1 showed increased expression (p ≤ 0.05) only in the tolerant genotype (vegetative stage/leaf and root) under water restriction. The different patterns of SOD activity and/or gene expression in upland rice plants should be strongly considered to elucidate the cellular mechanisms of drought tolerance; these findings may ultimately inform breeding programs aimed at more efficiently developing cultivars that are better adapted to areas prone to water deficits.
650 $aGene expression
650 $aOxidative stress
650 $aRice
650 $aSuperoxide dismutase
650 $aWater stress
650 $aArroz
650 $aOryza sativa
650 $aResistencia a seca
653 $aEnzymatic activity
700 1 $aLANNA, A. C.
700 1 $aABREU, F. R. M.
700 1 $aSILVEIRA, R. D. D.
700 1 $aPEREIRA, W. J.
700 1 $aBRONDANI, C.
700 1 $aVIANELLO, R. P.
773 $tAustralian Journal of Crop Science$gv. 9, n. 8, p. 744-753, 2015.
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Embrapa Arroz e Feijão (CNPAF) |
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